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Shaker, Olfat Gamil
- Assessment of the role of T Helper 17 cells in the pathogenesis of Acne Vulgaris
Authors
1 Cairo University, EG
2 Medical Biochemistry and Molecular Biology, Cairo University, EG
Source
Indian Journal of Public Health Research & Development, Vol 10, No 12 (2019), Pagination: 1435-1439Abstract
Background: Acne vulgaris, a multi-factorial disease, is considered one of the most common dermatological diseases carrying with it significant psychological disturbance. The mechanisms of inflammation occurring in acne are now a subject of intense investigation. The T-lymphocyte function is altered in AV together with the cytokines produced by T-cell subsets, hence implicated in the immunopathogenesis of AV. The role of T Helper 17 cells (Th17) in the pathogenesis of AV has not been established.
Objective: Study the level of expression of Th17 cytokine (IL-17) in tissue of patients of AV with both inflammatory and non-inflammatory lesions.
Method: 50 patients with AV as well as 30 age and sex matched healthy controls were included. Clinical examination was done and tissue levels of IL-17 were measured by ELISA.
Results: The mean tissue levels of IL-17 were significantly higher in patients than in controls (p < 0.001). A statistically significant relation was found between tissue levels of IL-17 and the type of lesion; where the mean levels of IL-17 were statistically significant in patients with both inflammatory (papules & pustules) and non-inflammatory type (comedones) of AV but was found statistically higher in inflammatory type.
Conclusion: Th17 cells potentially play a role in the pathogenesis of AV. Larger scale studies are required to verify the role of Th17 cells in inflammatory and non-inflammatory types of AV.
Keywords
Acne vulgaris – T Helper 17 - Pathogenesis – Inflammatory Lesions – Non-Inflammatory Lesions.
- lncRNAs as New Biomarkers in Systemic Lupus Erythematosus: A Prospective Study
Authors
1 Medical Biochemistry and Molecular Biology Department, Cairo University, EG
2 Rheumatology and Rehabilitation Department, Cairo University, EG
Source
Indian Journal of Public Health Research & Development, Vol 11, No 2 (2020), Pagination: 2509-2515Abstract
Aim: To investigate serum levels of two Immune-related functional lncRNAs, growth arrest-specific transcript 5 (GAS5) and metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), in Egyptian patients with SLE and to evaluate their relationship with disease activity.
Method: The present study was a case-control study that was carried out on 39 patients with SLE and 46 age and sex-matched healthy controls. The expression levels of GAS5 and MALAT1 were measured using by real-time polymerase chain reaction (PCR).
Results: There were statistically significant differences between cases and controls in GAS5 (p <0.001) and MALAT1 expression (p <0.01). The mean GAS5 was significantly higher in the control; while the mean MALATI expression was significantly higher in SLE patients. The ROC curve revealed that GAS5 was a good discriminant with AUC 0.849 with sensitivity 93.5% and specificity 74.3%. Moreover, MALAT1 was a good discriminant to differentiate cases from controls with AUC 0.3 with 95% CI (0.162 - 0.438), the most suitable cut-off point was ≥ 2.1 with Sensitivity 93.5% and Specificity 72.5%.
Conclusion: GAS5 and MALAT1 may serve as potential biomarkers for the diagnosis and monitoring of the SLE, both lnRNAs exhibited a good diagnostic accuracy to discriminate between SLE patients and healthy controls.